Drug Combination Details

General Information of the Combination (ID: C53594)
Name Sulforaphane   NP Info  + Temozolomide   Drug Info 
Structure +
Disease
Brain cancer [ICD-11: 2A00]
Investigative [1]
Combinatorial Therapeutic Effect(s) Validated Clinically or Experimentally
    α. Enhancing Drug Efficacy by This Combination
                 Achieving Therapeutic Synergy     Click to Show/Hide
                    Experiment 1 Reporting the Effect of This Combination [2]
                    Molecule(s)
                    Regulation		 Down-regulation Expression CTNNB1  Molecule Info 
Pathway MAP
Down-regulation Expression microRNA 21  Molecule Info 
Pathway MAP
Down-regulation Expression TCF7L2  Molecule Info 
Pathway MAP
Down-regulation Expression WNT1  Molecule Info 
Pathway MAP
                    In-vitro Model H4 CVCL_1239 Astrocytoma Homo sapiens
SNB-19 CVCL_0535 Glioblastoma Homo sapiens
LN-229 CVCL_0393 Glioblastoma Homo sapiens
U-251MG CVCL_0021 Astrocytoma Homo sapiens
                    Experimental
                    Result(s)		 SFN enhances TMZ-induced apoptosis in GBM cells by down-regulation of miR-21.
    β. Reversing Drug Resistance by This Combination
                 Reversing Drug Resistance     Click to Show/Hide
                    Experiment 1 Reporting the Effect of This Combination [1]
                    Molecule(s)
                    Regulation		 Down-regulation Expression MGMT  Molecule Info 
Pathway MAP
Down-regulation Expression p105  Molecule Info 
Pathway MAP
                    In-vitro Model U-87MG ATCC CVCL_0022 Glioblastoma Homo sapiens
U-373MG ATCC CVCL_2219 Astrocytoma Homo sapiens
T98G CVCL_0556 Glioblastoma Homo sapiens
                    In-vivo Model Nude mouse models were established with U373-R GBM cells inoculated subcutaneously into the right flanks of 4-6-week-old female mice.
                    Experimental
                    Result(s)		 SFN effectively inhibited activity of NF-KappaB signaling pathway and then reduced MGMT expression to reverse the chemo-resistance to TMZ in T98G, U87-R and U373-R cell lines.
References
Reference 1 Sulforaphane reverses chemo-resistance to temozolomide in glioblastoma cells by NF-KappaB-dependent pathway downregulating MGMT expression. Int J Oncol. 2016 Feb;48(2):559-68.
Reference 2 Sulforaphane enhances temozolomide-induced apoptosis because of down-regulation of miR-21 via Wnt/Beta-catenin signaling in glioblastoma. J Neurochem. 2015 Sep;134(5):811-8.
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